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Medium Detail Information

Medium No.
Medium for Methylotrophs
KNO3 1 g
MgSO4·6H2O 1 g
CaCl2·2H2O 0.2 g
Trace element solution* 1 ml
Phosphate buffer** 10 ml
Methanol 10 ml
Distilled water 1 L

Mix ingreginents except phophate buffer and methanol, adjust pH to 6.8, and then autoclave (121°C, 20 min). After autoclaving, add autoclaved phophate buffer and filter-sterile methanol. Final pH is 6.8.

**Trace element solution

Nitrilotriacetic acid (NTA) 12.8 g
FeCl3·6H2O 1.35 g
MnCl2·4H2O 0.1 g
CoCl2·6H2O 0.024 g
CaCl2·2H2O 0.1 g
ZnCl2 0.1 g
CuCl2·2H2O 0.025 g
H3BO3 0.01 g
Na2MoO4·2H2O 0.25 g
NaCl 1 g
NiCl2·6H2O 0.12 g
Distilled water 1 L

First dissolve NTA and adjust pH to 6.5 with NaOH, and then add minerals. Final pH is 7.0.

**Phosphate buffer

Na2HPO4·12H2O 71.6 g
KH2PO4 26 g
Distilled water 1 L