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Medium No.	1574
Medium	Brain Heart Infusion (BHI) with Sodium Acetate
Composition
Bacto Brain Heart Infusion (BD Difco) 37 g   Sodium acetate 2.7 g   Antifoam solution (only for liquid medium)* 2.5 mL   Distilled water 1 L   Agar (if needed) 15 g   pH unadjusted         For liquid medium: Dispense the medium into suitable culture vessels and autoclave at 121°C for 15 minutes under a N2 atomosphere. For agar plate: Sterilizing by autoclaving at 121°C for 15 minutes. After autoclaving, cool to about 50°C, and dispense into sterile petri dishes.                   *Antifoam solution       Antifoam SI (Wako) 1 mL       Distiled water 9 mL
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