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Medium No.
Brain Heart Infusion (BHI) with Sodium Acetate
  Bacto Brain Heart Infusion (BD Difco) 37 g
  Sodium acetate 2.7 g
  Antifoam solution (only for liquid medium)* 2.5 mL
  Distilled water 1 L
  Agar (if neded) 15 g
  pH unadjusted  
    For liquid medium: Dispense the medium into suitable culture vessels and autoclave at 121°C for 15 minutes under a N2 atomosphere.
For agar plate: Sterilizing by autoclaving at 121°C for 15 minutes. After autoclaving, cool to about 50°C, and dispense into sterile petri dishes.
    *Antifoam solution
      Antifoam SI (Wako) 1 mL
      Distiled water 9 mL