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Medium Detail Information

Medium No.
1579
Medium
BNB medium
Composition
  Solution A* 500 ml
  Solution B** 500 ml
       
    Solutions A and B are autoclaved separately. After the solutions are cooled down at 50-60°C, mixed each other.
     
    *Solution A
      (NH4)2SO4 2.0 g
      KCl 0.1 g
      KH2PO4 0.5 g
      MgSO4·7H2O 0.5 g
      Ca(NO3)·4H2O 10 mg
      Yeast extract 0.1 g
      Distilled water 500 ml
  Adjust pH to 3.5 with H2SO4  
     
    **Solution B
      Nutrient Broth 2.0 g
      Agar 20 g
      Distilled water 500 ml
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