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Medium Detail Information

Medium No.
Medium for nitrite oxidizer
NaNO2 49.2-98.6 mg
K2HPO4 25.4 mg
MgSO4·7H2O 40.6 mg
CaCl2·2H2O 6.6 mg
FeSO4·7H2O 3.2 mg
NaHCO3 200 mg
MnSO4·5H2O 54.2 μg
H3BO3 49.4 μg
ZnSO4·7H2O 43.1 μg
Na2Mo4O4 27.6 μg
CuSO4·5H2O 25.0 μg
Distilled water 1 L

Mix ingregients except NaNO2, K2HPO4, MgSO4·7H2O, CaCl2·2H2O, FeSO4·7H2O, and NaHCO3, dispense the medium into suitable culture vessels, and autoclave (121°C, 20min). NaNO2, K2HPO4, MgSO4·7H2O, CaCl2·2H2O are autoclaved separately, and added to the medium. Filter-sterile FeSO4·7H2O and NaHCO3 solutions are added to the medium. Final pH is 7.8-8.3.