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Medium Detail Information

Medium No.
 1683
Medium
 MOORELLA MEDIUM (DSMZ 60)
Composition

Solution A 843 ml
Solution B 50 ml
Solution C 50 ml
Solution D 40 ml
Solution E 10 ml
Solution F 10 ml
pH 6.9  

Autoclave solution A in Hungate-type tubes or serum vials under 100% CO2 or 80% N2 and 20% CO2 gas atmosphere. Autoclave separately solutions B, C, E and F under 100% N2 gas, and solution D under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of the complete medium to 6.9, if necessary.
Solution A 
MgSO4 x 7 H2O 0.10 g
(NH4)2SO4 0.50 g
Fe(NH4)2(SO4)2              0.04 g
Na2MoO4 x 2 H2O (0.1% w/v) 2.40 ml
Na2SeO3 x 5 H2O (0.1% w/v) 0.15 ml
Tryptone 5.00 g
Yeast extract 5.00 g
Sodium resazurin (0.1% w/v) 0.50 ml
Distilled water 840.00 ml
     
Solution B
K2HPO4    7.00 g
KH2PO4  4.50 g
Distilled water 50.00 ml
     
Solution C
Glucose 18.00 g
Distilled water
 50.00 ml
     
Solution D
Na2CO3
 2.00 g
Distilled water
 40.00 ml
     
Solution E 
L-Cysteine HCl x H2O
 0.30 g
Distilled water 
 10.00 ml
     
Solution F 
Na2S x 9 H2O 0.30 g
Distilled water  10.00 ml





















































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