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Medium Detail Information

Medium No.	1712
Medium
Composition
Solution A* 500  ml   Solution B** 500  ml         pH 4.0                 Sterilize Solutions A and B separately by autoclaving, then mixed them. Add 1 ml of Trace Elements Solution*** and adjust the pH to 4.0.                 *Solution A CaCl2·2H2O 0.25  g       MgSO4·7H2O 0.5  g       (NH4)2SO4 0.2  g       KH2PO4 3  g       Distilled water 500  ml         pH 4.0                   **Solution B             Glucose 5  g       Yeast extract 2  g       Agar (if needed) 15  g       Distilled water 500  ml                 ***Trace Elements solution             ZnSO4·7H2O 0.1  g       MnCl2·4H2O 0.03  g       H3BO3 0.3  g       CoCl2·6H2O 0.2  g       CuCl2·2H2O 0.01  g       NiCl2·6H2O 0.02  g       Na2MoO4·2H2O 0.03  g       Distilled water 1  L
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