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Medium Detail Information

Medium No.	221
Medium
Composition
Uric acid 2 g   KOH 0.67 g   K2HPO4 1 g   MgSO4·7H2O 35 mg   FeSO4·7H2O 35 mg   CaCl2·2H2O 4.2 mg   Yeast extract 1 g   Mercaptoacetic acid (80% solution) 2 ml   Distilled water 1 L   Agar (if needed) 15 g   pH 7.0       First dissolve KOH and K2HPO4 in water, then add uric acid and boil until the uric acid is dissolved. Add the metal salts followed by yeast extract, which should be first dissolved in the little water. Add the mercaptoacetic acid and adjust the pH to 7.0-7.2. Distribute the medium into culture vessels under a stream of N2/CO2 (80/20) gas (or N2 gas) and seal with butyl rubber stoppers, and then autoclave it. Store the medium at 37℃ until required to prevent precipitation of the uric acid.
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