National Institute of Technology and Evaluation
NITE Biological Resource Center
Biotechnology Center
HOME > NBRC > NBRC Culture Collection > NBRC Culture Catalogue Search > Catalogue Search Result

Medium Detail Information

Medium No.
267 
Medium
Modified Hayflick Medium 
Composition
 Bacto Heart Infusion Broth (Difco)25g
 Distilled water700ml
 Bacto Agar (Difco, if needed)15g
 pH 7.8 
  Sterilized by autoclaving (121℃ for 20 min).
  Aseptically add 200 ml of horse serum (heat inactivated, 56℃ for 30 minutes), 100 ml of fresh yeast extract (25% solution)*, 12 ml of calf thymus DNA (Sigma, Type I) (0.2% (w/v) solution), 10 ml of thallium acetate (1% (w/v) solution), 25 ml of Penicillin G (20,000 IU/ml).
       
  *Boil 250 g of dried baker's yeast suspended in 1 L of distilled water for 30 min, remove yeast cells by centrifugation, and sterilize by filtration.
 
Comment
 


contact us
Disclaimer Terms of use
National Institute of Technology and Evaluation (JCN 9011005001123)
2-49-10 Nishihara, Shibuya-ku, Tokyo, Japan