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Medium Detail Information

Medium No.
Modified Hayflick Medium
 Bacto Heart Infusion Broth (Difco)25g
 Distilled water700ml
 Bacto Agar (Difco, if needed)15g
 pH 7.8 
  Sterilized by autoclaving (121℃ for 20 min).
  Aseptically add 200 ml of horse serum (heat inactivated, 56℃ for 30 minutes), 100 ml of fresh yeast extract (25% solution)*, 12 ml of calf thymus DNA (Sigma, Type I) (0.2% (w/v) solution), 10 ml of thallium acetate (1% (w/v) solution), 25 ml of Penicillin G (20,000 IU/ml).
  *Boil 250 g of dried baker's yeast suspended in 1 L of distilled water for 30 min, remove yeast cells by centrifugation, and sterilize by filtration.