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Medium Detail Information

Medium No.
Ekho Lake Strains Medium
 Mineral salt solution* ("Hutner/Cohen-Bazire")20ml
 Bacto Peptone (Difco)0.25g
 Bacto Yeast Extract (Difco)0.25g
 Bacto Agar (Difco)15g
 Glucose solution (2.5%, filter-sterilized)10ml
 Vitamin solution**5ml
 Artificial sea water***250ml
 Distilled water715ml
 pH 7.5 
  Mix ingredients (except glucose and vitamin solutions), and sterilize 20 min at 121℃. After cooling to 60℃, aseptically add glucose and vitamin solutions to the medium. The medium is only weakly buffered; one needs approximately 10 drops/1 medium of 6 N KOH.
  *Mineral salt solution
   Nitrilotriacetic acid (NTA)10g
   Metal salt solution 44****50ml
   Distilled water900ml
  Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1 L with distilled water.
  **Vitamin solution
   Folic acid4mg
   Vitamin B120.2mg
   p-Aminobenzoic acid10mg
   Distilled water1L
  ***Artificial sea water
   Distilled water1L
  ****Metal salt solution 44
   Distilled water100ml
  Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. Store in the dark and cold (5℃).