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Medium Detail Information

Medium No.
Medium for Tepidanaerobacter
 Yeast extract2.3g
 Vitamin solution*2ml
 Trace element solution**1ml
 Se/W solution***1ml
 Resazurin solution****1ml
 Distilled water1L
  *Vitamin solution
   Folic acid0.9mg
   Nicotinic acid0.2mg
   Vitamin B122.7mg
   p-Aminobenzoic acid0.3mg
   Lipoic acid0.4mg
   Distilled water1L
  **Trace element solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve NTA in 200 ml of water and adjust the pH to 6.5 with KOH.
  ***Se/W solution
  ****Resazurin solution
   Distilled water1L
  For vial cultures, dissolve all ingredients except for glucose, yeast extract , Na2S·9H2O and cysteine-HCl (up to 900 ml), flush N2/CO2 (80/20) (pH 7), dispense 18 ml into 50-ml vials, fix with flushing N2/CO2 (80/20) and autoclave. Add 0.5 ml of each of filter-sterilized Na2S·9H2O solution (0.6 g Na2S·9H2O/50 ml) and cysteine-HCl solution (0.6 g cysteine-HCl/ 50ml) separately. Add 0.5 ml of neutralized and filter sterilized 0.8 M glucose solution and 0.5 ml of autoclaved yeast extract solution (%). Inoculum size 10%.