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Medium Detail Information

Medium No.
391
Medium
Thermodesulfobium narugense Medium
Composition
  K2HPO4 0.78 g
  KH2PO4 0.75 g
  3Na-EDTA 0.04 g
  FeSO4·7H2O 0.01 g
  MgSO4·7H2O 0.25 g
  CaCl2·2H2O 0.03 g
  NaCl 0.2 g
  NH4Cl 0.5 g
  Na2SO4 20 mM
  Sodium acetate 2 mM
  Vitamin solution* 10 ml
  Trace element solution** 10 ml
  Cysteine-HCl 0.25 g
  Distilled water 1 L
    For vial culture, dissolve all ingredients except cysteine-HCl, flush N2/CO2, dispense 20 ml/50-ml vials, fix with a stopper with flushing N2/CO2 and autoclave. After autoclaved, the gas phase is replaced with H2/CO2 (80/20, 150 kPa), and add 0.1 ml of autoclaved cysteine-HCl solution (50 g/l) per each vial separately. Inoculum size 5-10%.
             
    *Vitamin solution
      Biotin 2 mg
      Folic acid 2 mg
      Pyridoxine-HCl 10 mg
      Thiamine-HCl 5 mg
      Riboflavin 5 mg
      Nicotinic acid 5 mg
      Ca-pantothenate 5 mg
      Vitamin B12 0.1 mg
      p-Aminobenzoic acid 5 mg
      Lipoic acid 5 mg
      Distilled water 1 L
             
    **Trace element solution
      Nitrilotriacetic acid (NTA) 12.8 g
      FeCl3·6H2O 1.35 g
      MnCl2·4H2O 0.1 g
      CoCl2·6H2O 0.024 g
      CaCl2·2H2O 0.1 g
      ZnCl2 0.1 g
      CuCl2·2H2O 0.025 g
      H3BO3 0.01 g
      Na2MoO4·2H2O 0.024 g
      NaCl 1 g
      NiCl2·6H2O 0.12 g
      Na2SeO3·5H2O 0.026 g
      Distilled water 1 L
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