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Medium No.	391
Medium	Thermodesulfobium narugense Medium
Composition
K2HPO4 0.78 g   KH2PO4 0.75 g   3Na-EDTA 0.04 g   FeSO4·7H2O 0.01 g   MgSO4·7H2O 0.25 g   CaCl2·2H2O 0.03 g   NaCl 0.2 g   NH4Cl 0.5 g   NaSO4 20 mM   Sodium acetate 2 mM   Vitamin solution* 10 ml   Trace element solution** 10 ml   Cysteine-HCl 0.25 g   Distilled water 1 L     For vial culture, dissolve all ingredients except cysteine-HCl, flush N2/CO2, dispense 20 ml/50-ml vials, fix with a stopper with flushing N2/CO2 and autoclave. After autoclaved, the gas phase is replaced with H2/CO2 (80/20, 150 kPa), and add 0.1 ml of autoclaved cysteine-HCl solution (50 g/l) per each vial separately. Inoculum size 5-10%.                   *Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       Vitamin B12 0.1 mg       p-Aminobenzoic acid 5 mg       Lipoic acid 5 mg       Distilled water 1 L                   **Trace element solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Na2SeO3·5H2O 0.026 g       Distilled water 1 L
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