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Medium Detail Information

Medium No.
Thermodesulfobium narugense Medium
 Sodium acetate2mM
 Vitamin solution*10ml
 Trace element solution**10ml
 Distilled water1L
  For vial culture, dissolve all ingredients except cysteine-HCl, flush N2/CO2, dispense 20 ml/50-ml vials, fix with a stopper with flushing N2/CO2 and autoclave. After autoclaved, the gas phase is replaced with H2/CO2 (80/20, 150 kPa), and add 0.1 ml of autoclaved cysteine-HCl solution (50 g/l) per each vial separately. Inoculum size 5-10%.
  *Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
  **Trace element solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L