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Medium Detail Information

Medium No.
Archaeoglobus Medium
 Bacto Yeast Extract (Difco)0.5g
 Sodium lactate1.5g
 Trace element solution*10ml
 Distilled water1L
  Dissolve ingredients except bicarbonate and sulfide, bring to boil for few minutes, then cool quickly to room temperature while gassing with N2/CO2 (80/20), add sodium bicarbonate and adjust pH to 6.9. Distribute in serum bottles (20 ml per 100 ml bottle) under N2/CO2, seal, pressurize bottles up to 2 bar overpressure, then autoclave. Before use, reduce the medium with sodium sulfide from anaerobic sterile neutral stock solution.
  *Trace element solution
   Nitrilotriacetic acid1.5g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).