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Medium Detail Information

Medium No.
394
Medium
Pyrococcus Medium 2
Composition
 Salt base solution*1L
 Trace minerals**10ml
 Citric acid5mg
 Bacto Yeast Extract (Difco)1g
 Bacto Peptone (Difco)5g
 Sulfur (powder)***30g
 Na2S·9H2O0.5g
 Resazurin1mg
  Mix ingredients except sulfur and Na2S·9H2O, and adjust pH to 6.5 with H2SO4. Sterilize by filtration under a N2 atmosphere. Prepare Na2S·9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution, and readjust pH to 6.5, if necessary.
       
  *Salt base solution
   NaCl13.85g
   MgSO4·7H2O3.5g
   MgCl2·6H2O2.75g
   KCl0.325g
   NaBr0.05g
   H3BO315mg
   SrCl2·6H2O7.5mg
   KI0.05mg
   CaCl20.75g
   KH2PO40.5g
   (NH4)2Ni(SO4)2·6H2O2mg
   Distilled water1L
       
  **Trace minerals
   Nitrilotriacetic acid1.5g
   MgSO4·7H2O3g
   MnSO4·xH2O0.5g
   NaCl1g
   FeSO4·7H2O0.1g
   CoSO4·7H2O0.1g
   CaCl2·2H2O0.1g
   ZnSO4·7H2O0.1g
   CuSO4·5H2O0.01g
   AlK(SO4)20.01g
   H3BO30.01g
   Na2MoO4·2H2O0.01g
   Distilled water1L
  Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
       
  ***Sulfur powder
  Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days.
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