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Medium Detail Information

Medium No.
Pyrococcus Medium 2
 Salt base solution*1L
 Trace minerals**10ml
 Citric acid5mg
 Bacto Yeast Extract (Difco)1g
 Bacto Peptone (Difco)5g
 Sulfur (powder)***30g
  Mix ingredients except sulfur and Na2S·9H2O, and adjust pH to 6.5 with H2SO4. Sterilize by filtration under a N2 atmosphere. Prepare Na2S·9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution, and readjust pH to 6.5, if necessary.
  *Salt base solution
   Distilled water1L
  **Trace minerals
   Nitrilotriacetic acid1.5g
   Distilled water1L
  Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
  ***Sulfur powder
  Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days.