カタログ検索結果 詳細情報

Medium Detail Information

Medium No.	405
Medium	Methanosarcina Medium
Composition
KH2PO4 0.227 g   K2HPO4 0.348 g   NH4Cl 0.5 g   MgSO4·7H2O 0.5 g   CaCl2·2H2O 0.25 g   NaCl 2.25 g   FeSO4·7H2O 2 mg   Bacto Yeast Extract (Difco) 2 g   Bacto Casitone (Difco) 2 g   Trace vitamins* 10 ml   FeCl2 solution** 1 ml   Trace element solution*** 1 ml   NaHCO3 0.85 g   Methanol 10 ml   Cysteine-HCl 0.3 g   Na2S·9H2O 0.3 g   Resazurin 1 mg   Distilled water 980 ml     Mix ingredients except NaHCO3, methanol, cysteine-HCl and Na2S·9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of N2/CO2 (80/20), and add NaHCO3 and methanol to the medium. Dispense the medium into suitable culture vessels (e.g. 20 ml of the medium in 120 ml serum bottles) under a stream of N2/CO2 (80/20) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine-HCl and Na2S·9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine-HCl and Na2S·9H2O solutions.                   *Trace vitamins       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       Vitamin B12 0.1 mg       p-Aminobenzoic acid 5 mg       Lipoic acid 5 mg       Distilled water 1 L                   **FeCl2 solution       25% HCl (7.7 M) 10 ml       FeCl2·4H2O 1.5 g       Distilled water 990 ml                   ***Trace element solution       ZnCl2 70 mg       MnCl2·4H2O 100 mg       H3BO3 6 mg       CoCl2·6H2O 190 mg       CuCl2·2H2O 2 mg       NiCl2·6H2O 24 mg       Na2MoO4·2H2O 36 mg       Distilled water 1 L
Comment