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Medium Detail Information

Medium No.
Methanosarcina Medium
 Bacto Yeast Extract (Difco)2g
 Bacto Casitone (Difco)2g
 Trace vitamins*10ml
 FeCl2 solution**1ml
 Trace element solution***1ml
 Distilled water980ml
  Mix ingredients except NaHCO3, methanol, cysteine-HCl and Na2S·9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of N2/CO2 (80/20), and add NaHCO3 and methanol to the medium. Dispense the medium into suitable culture vessels (e.g. 20 ml of the medium in 120 ml serum bottles) under a stream of N2/CO2 (80/20) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine-HCl and Na2S·9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine-HCl and Na2S·9H2O solutions.
  *Trace vitamins
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
  **FeCl2 solution
   25% HCl (7.7 M)10ml
   Distilled water990ml
  ***Trace element solution
   Distilled water1L