Search Results Detail

Medium Detail Information

Medium No.
Methanocaldococcus jannaschii Medium
 Trace elements solution*10ml
 Vitamin solution**10ml
 Distilled water1L
  Mix ingredients except vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under a H2/CO2 (80/20) atmosphere. Separately autoclave cysteine-HCl and Na2S·9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add filter-sterilized vitamin, cysteine-HCl and Na2S·9H2O. Pressurize the inoculated bottles to 200 kPa with H2/CO2 (80/20).
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L