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Medium Detail Information

Medium No.
810
Medium
Methanocaldococcus jannaschii Medium
Composition
 K2HPO40.14g
 CaCl2·2H2O0.14g
 NH4Cl0.25g
 MgSO4·7H2O3.4g
 MgCl2·6H2O2.7g
 KCl0.33g
 Na2SeO30.5mg
 NaCl30g
 Fe(NH4)2(SO4)2·6H2O1mg
 Trace elements solution*10ml
 Vitamin solution**10ml
 Resazurin1mg
 NaHCO31g
 Cysteine-HCl0.5g
 Na2S·9H2O0.5g
 Distilled water1L
  Mix ingredients except vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under a H2/CO2 (80/20) atmosphere. Separately autoclave cysteine-HCl and Na2S·9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add filter-sterilized vitamin, cysteine-HCl and Na2S·9H2O. Pressurize the inoculated bottles to 200 kPa with H2/CO2 (80/20).
       
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
       
  **Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
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