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Medium Detail Information

Medium No.
 Bacto PPLO Broth (Difco)21g
 Horse serum*100ml
 Phenol red0.02g
 Penicillin G** (20,000 U/ml)5ml
 Distilled water900ml
 pH 6.8-7.0 
  *Heat inactivated at 56℃ for 30 min.
  **Sterilized by filtration.
  Dissolve 21 g of PPLO broth, 3 g of L-arginine, and 0.02 g of phenol red in 900 ml of water (adjusted pH to 6.8-7.0. sterilized by autoclave at 121℃ for 15 min). Aseptically add 100 ml of horse serum and 5 ml of Penicillin G (200,000 U/ml, sterilized by filtration) to the cooled broth.