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Medium No.	832
Medium	Pyrodictium Medium
Composition
NaCl 13.85 g   MgSO4·7H2O 3.5 g   MgCl2·6H2O 2.75 g   KCl 0.325 g   NaBr 0.05 g   H3BO3 15 mg   SrCl2·6H2O 7.5 mg   KI 0.05 mg   CaCl2 0.75 g   KH2PO4 0.5 g   (NH4)2Ni(SO4)2·6H2O 2 mg   Trace minerals* 10 ml   Bacto Yeast Extract (Difco) 2 g   Citric acid 5 mg   Sulfur (powder) 30 g   Resazurin 1 mg   Na2S·9H2O 0.3 g   Distilled water 1 L     Mix ingredients except sulfur and Na2S·9H2O, adjust pH to 5.5 with H2SO4, and autoclave under H2/CO2 (80/20) conditions. Separately autoclave neutralize Na2S·9H2O solution. Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days. Aseptically and anaerobically add sterile sulfur to the medium under a H2/CO2 (80/20) atmosphere. Prior to inoculation, add Na2S·9H2O solution, and readjust pH to 5.5 if necessary. Pressurize inoculated bottles to 200 kPa with H2/CO2 (80/20).                   *Trace minerals       Nitrilotriacetic acid 1.5 g       MgSO4·7H2O 3 g       MnSO4·xH2O 0.5 g       NaCl 1 g       FeSO4·7H2O 0.1 g       CoSO4·7H20 0.1 g       CaCl2·2H2O 0.1 g       ZnSO4·7H2O 0.1 g       CuSO4·5H2O 0.01 g       AlK(SO4)2 0.01 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.01 g       Distilled water 1 L     Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
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