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Medium Detail Information

Medium No.
Pyrodictium Medium
 Trace minerals*10ml
 Bacto Yeast Extract (Difco)2g
 Citric acid5mg
 Sulfur (powder)30g
 Distilled water1L
  Mix ingredients except sulfur and Na2S·9H2O, adjust pH to 5.5 with H2SO4, and autoclave under H2/CO2 (80/20) conditions. Separately autoclave neutralize Na2S·9H2O solution. Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days. Aseptically and anaerobically add sterile sulfur to the medium under a H2/CO2 (80/20) atmosphere. Prior to inoculation, add Na2S·9H2O solution, and readjust pH to 5.5 if necessary. Pressurize inoculated bottles to 200 kPa with H2/CO2 (80/20).
  *Trace minerals
   Nitrilotriacetic acid1.5g
   Distilled water1L
  Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.