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Medium Detail Information

Medium No.
Thermoproteus tenax Medium
 Bacto Yeast Extract (Difco)0.2g
 Soluble starch5g
 Sulfur (powder)10g
 Distilled water1L
  Mix ingredients except yeast extract, starch, sulfur and Na2S·9H2O, and filter-sterilize while gassing with N2. Autoclave yeast extract and starch as 10% solutions and Na2S·9H2O as a 5% solution under a N2 atmosphere. Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days. Aseptically and anaerobically add the yeast extract and starch solutions, and sulfur to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution, and adjust pH to 5.5 with sterile 1 N H2SO4.