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Medium Detail Information

Medium No.
Thermoproteus neutrophilus Medium
 Bacto Yeast Extract (Difco)0.2g
 Sulfur (powder)8g
 Distilled water1L
  Mix ingredients except yeast extract, NaHCO3, Na2S2O4 and sulfur, and adjust pH to 6.5. Autoclave, then cool under a H2/CO2 (80/20) atmosphere. Separately autoclave yeast extract as a 10% solution under a N2 atmosphere. Filter-sterilize NaHCO3 as a 8% solution while gassing with N2. Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days. Aseptically and anaerobically add the yeast extract and NaHCO3 solutions, and sulfur to the medium under a H2/CO2 (80/20) atmosphere. Readjust pH to 6.5 by further gassing with H2/CO2 (80/20) gas mixture, if necessary. Prior to inoculation, add filter-sterilized 2.5% Na2S2O4 solution. Pressurize inoculated bottles to 150 kPa with H2/CO2 (80/20).