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Medium Detail Information

Medium No.
Methanosarcina Medium
 Bacto Yeast Extract (Difco)0.1g
 Sodium acetate2.5g
 Trace mineral solution**9ml
 Trace vitamins***10ml
 Distilled water981ml
  Mix ingredients except trace vitamins, Na2S·9H2O and cysteine-HCl, and autoclave under a N2/CO2 (80/20) atmosphere. Separately autoclave Na2S·9H2O and cysteine-HCl as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add filter-sterile vitamin solution to the medium. Prior to inoculation, add the sterile Na2S·9H2O and cysteine-HCl solutions, and adjust pH to 6.8.
  *Wako Pure Chemicals Ind. Ltd., Osaka, Japan.
  **Trace mineral solution
   Nitrilotriacetic acid4.5g
   Distilled water1L
 Adjust pH to 7.0 with 1 N KOH 
  ***Trace vitamins
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L