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Medium Detail Information

Medium No.
Thermoproteus tenax Medium 2
 Bacto Yeast Extract (Difco)0.2g
 Soluble starch5g
 Sulfur (powder)8g
 Distilled water1L
  Mix ingredients except NaHCO3, yeast extract, soluble starch, Na2S·9H2O and sulfur, and adjust pH to 5.5. Autoclave, then cool under N2/CO2 (80/20) gas mixture. Under a N2 atmosphere, separately autoclave yeast extract, soluble starch and Na2S·9H2O as 2%, 10% and 5% solutions, respectively. Filter-sterilize NaHCO3 as a 8% solution while gassing with N2. Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days. Aseptically and anaerobically add the yeast extract, soluble starch and NaHCO3 solutions, and sulfur to the medium under a N2/CO2 (80/20) atmosphere. Readjust pH to 5.5, if necessary. Prior to inoculation, add Na2S·9H2O.