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Medium No.
Mycoplasma Medium with Sucrose
 Bacto Heart Infusion Broth (Difco)17.5g
 Horse serum*200ml
 15% Yeast Extract Solution (Gibco 0689)150ml
 Phenol red40mg
 Distilled water650ml
  *Heat-inactivated at 56℃ for 30 min.
  Dissolve 17.5 g of heart infusion broth and 40 mg of phenol red in 450 ml of water (sterilized by autoclaving at 121℃ for 15 min). Dissolve sucrose in 200 ml of water (sterilized by filtration). Aseptically add the horse serum, yeast extract solution, sterile sucrose, and 1 ml of Penicillin G (200,000 U/ml, sterilized by filtration) to the cooled heart infusion broth. Final pH 7.4 +/- 0.2.