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Medium Detail Information

Medium No.	844
Medium	Pyrococcus Medium
Composition
NaCl 13.5 g   Na2SO4 4 g   MgCl2·6H2O 10.8 g   CaCl2·2H2O 1.5 g   Yeast extract 1 g   Tryptone 5 g   Resazurin 1 mg   Solution 1* 10 ml   Solution 2** 10 ml   Sulfur (powder) 30 g   Na2S·9H2O 0.25 g   Distilled water 1 L     Mix ingredients except solutions 1 and 2, sulfur, and Na2S·9H2O, and autoclave under a N2 atmosphere. Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days. Neutralize Na2S·9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur and filter-sterile solutions 1 and 2 to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution.                   *Solution 1       KCl 7 g       NaHCO3 2 g       NaBr 1 g       Distilled water 1 L                   **Solution 2       H3BO3 0.3 g       SrCl2·6H2O 0.25 g       Distilled water 1 L
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