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Medium Detail Information

Medium No.
Thermoproteus and Vulcanisaeta medium
 Bacto Yeast Extract (Difco)1g
 Sulfur (powder)5g
 Distilled water1L
  Mix ingredients except yeast extract, sulfur and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave yeast extract as a 10% solution and Na2S·9H2O as a 5% solution under a N2 atmosphere. Sulfur is autoclaved for 60 min at 105℃ on each of 3 successive days. Aseptically and anaerobically add yeast extract and sulfur to the medium. Prior to inoculation, add sterile Na2S·9H2O solution, and adjust pH to 4.5-5.0 with sterile 1 N H2SO4.