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Medium Detail Information

Medium No.	847
Medium	Thermoproteus and Vulcanisaeta medium
Composition
(NH4)2SO4 1.3 g   KH2PO4 0.28 g   MgSO4·7H2O 0.25 g   CaCl2·2H2O 0.07 g   FeCl3·6H2O 0.02 g   MnCl2·4H2O 1.8 mg   Na2B4O7·10H2O 4.5 mg   ZnSO4·7H2O 0.22 mg   CuCl2·2H2O 0.05 mg   Na2MoO4·2H2O 0.03 mg   VOSO4·nH2O 0.03 mg   CoSO4·7H2O 0.01 mg   Bacto Yeast Extract (Difco) 1 g   Sulfur (powder) 5 g   Na2S·9H2O 0.5 g   Resazurin 1 mg   Distilled water 1 L     Mix ingredients except yeast extract, sulfur and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave yeast extract as a 10% solution and Na2S·9H2O as a 5% solution under a N2 atmosphere. Sulfur is autoclaved for 60 min at 105℃ on each of 3 successive days. Aseptically and anaerobically add yeast extract and sulfur to the medium. Prior to inoculation, add sterile Na2S·9H2O solution, and adjust pH to 4.5-5.0 with sterile 1 N H2SO4.
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