Search Results Detail

Medium Detail Information

Medium No.
847
Medium
Thermoproteus and Vulcanisaeta medium
Composition
 (NH4)2SO41.3g
 KH2PO40.28g
 MgSO4·7H2O0.25g
 CaCl2·2H2O0.07g
 FeCl3·6H2O0.02g
 MnCl2·4H2O1.8mg
 Na2B4O7·10H2O4.5mg
 ZnSO4·7H2O0.22mg
 CuCl2·2H2O0.05mg
 Na2MoO4·2H2O0.03mg
 VOSO4·nH2O0.03mg
 CoSO4·7H2O0.01mg
 Bacto Yeast Extract (Difco)1g
 Sulfur (powder)5g
 Na2S·9H2O0.5g
 Resazurin1mg
 Distilled water1L
  Mix ingredients except yeast extract, sulfur and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave yeast extract as a 10% solution and Na2S·9H2O as a 5% solution under a N2 atmosphere. Sulfur is autoclaved for 60 min at 105℃ on each of 3 successive days. Aseptically and anaerobically add yeast extract and sulfur to the medium. Prior to inoculation, add sterile Na2S·9H2O solution, and adjust pH to 4.5-5.0 with sterile 1 N H2SO4.
Comment