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Medium Detail Information

Medium No.
Modified MB Medium
 Yeast extract1g
 Bacto Tryptone (Difco)2g
 Soluble starch10g
 Trace element solution*10ml
 Distilled water1L
 Adjust pH to 7.5 with 2 M NaOH 
  Dissolve ingredients except thiosulfate and cysteine-HCl, boil medium for 3 min, then cool to room temperature under a N2 atmosphere, and add cysteine-HCl. Adjust pH to 7.5, dispense under same gas atmosphere in culture vessels and autoclave. Before inoculation, add thiosulfate from a sterile, anaerobic stock solutions prepared under N2.
  *Trace element solution
   Nitrilotriacetic acid1.5g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).