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Medium Detail Information

Medium No.
851
Medium
Thermotoga maritima Medium
Composition
 Soluble starch5g
 KH2PO40.5g
 Trace minerals*10ml
 NaCl20g
 Bacto Yeast Extract (Difco)0.5g
 Resazurin1mg
 Na2S·9H2O**0.5g
 Artificial sea water***250ml
 Distilled water750ml
  Mix ingredients except Na2S·9H2O, adjust pH to 6.5 with NaOH, and autoclave under a N2 atmosphere. Separately autoclave and neutralize Na2S·9H2O as 5% solution under a N2 atmosphere. Anaerobically add Na2S·9H2O solution.
       
  *Trace minerals
   Nitrilotriacetic acid1.5g
   MgSO4·7H2O3g
   MnSO4·2H2O0.5g
   NaCl1g
   FeSO4·7H2O0.1g
   CoSO4·7H2O0.18g
   CaCl2·2H2O0.1g
   ZnSO4·7H2O0.18g
   CuSO4·5H2O0.01g
   H3BO30.01g
   Na2MoO4·2H2O0.01g
   NiCl2·6H2O0.025g
   Na2SeO3·5H2O0.8mg
   Na2WO40.8mg
   KAl(SO4)2·12H2O4mg
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
       
  **The sulfide is prepared in a vessel sealed with a butyl rubber stopper. Autoclave under a N2 atmosphere. After cooling, the pH is adjusted to about 7.3 by adding of sterile 4-0.2 N H2SO4 drop-wise with a syringe without opening the bottle. Appearance of yellow color indicate the pH is about 8. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow.
       
  ***Artificial sea water
   NaCl27.7g
   MgSO4·7H2O7g
   MgCl2·6H2O5.5g
   CaCl2·2H2O2.25g
   KCl0.65g
   NaBr0.1g
   H3BO330mg
   SrCl2·6H2O15mg
   Citric acid10mg
   KI0.05mg
   Distilled water1L
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