カタログ検索結果 詳細情報

Medium Detail Information

Medium No.	852
Medium	Pyrobaculum aerophilum Medium
Composition
Marine medium/synthetic seawater mix solution* 125 ml   Trace elements** 10 ml   NH4Cl 0.25 g   NaHCO3 2.2 g   KH2PO4 0.07 g   Fe(NH4)2(SO4)2·6H2O 2 mg   (NH4)2Ni(SO4)2·6H2O 2 mg   Bacto Yeast Extract (Difco) 0.5 g   Na2S2O3·5H2O 1 g   Distilled water 865 ml     After flushing with N2/CO2, adjust pH to 7.0 with 1 N H2SO4. Sterilize by filtration. Dispense the medium into vessels under a N2/CO2/O2 (79/20/1) atmosphere. Pressurize the inoculated vessels to 200 kPa with N2/CO2/O2.                   *Marine medium/synthetic seawater mix solution       NaCl 47 g       MgCl2·6H2O 18 g       MgSO4·7H2O 7 g       CaCl2·2H2O 3 g       Na2SO4 3.13 g       Na2CO3 0.1 g       NaBr 0.2 g       SrCl2·6H2O 72 mg       H3BO3 52 mg       Na2HPO4 8.1 mg       NaF 2.4 mg       KI 0.05 mg       Sodium silicate 0.4 g       Distilled water 1 L                   **Trace elements       Nitrilotriacetic acid 1.5 g       MgSO4·7H2O 3 g       MnSO4·2H2O 0.5 g       NaCl 1 g       FeSO4·7H2O 0.1 g       CoSO4·7H2O 0.18 g       CaCl2·2H2O 0.1 g       ZnSO4·7H2O 0.18 g       CuSO4·5H2O 0.01 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.01 g       NiCl2·6H2O 0.025 g       Na2SeO3·5H2O 0.4 mg       Na2WO4 0.4 mg       KAl(SO4)2·12H2O 2 mg       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
Comment