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Medium Detail Information

Medium No.
852
Medium
Pyrobaculum aerophilum Medium
Composition
 Marine medium/synthetic seawater mix solution*125ml
 Trace elements**10ml
 NH4Cl0.25g
 NaHCO32.2g
 KH2PO40.07g
 Fe(NH4)2(SO4)2·6H2O2mg
 (NH4)2Ni(SO4)2·6H2O2mg
 Bacto Yeast Extract (Difco)0.5g
 Na2S2O3·5H2O1g
 Distilled water865ml
  After flushing with N2/CO2, adjust pH to 7.0 with 1 N H2SO4. Sterilize by filtration. Dispense the medium into vessels under a N2/CO2/O2 (79/20/1) atmosphere. Pressurize the inoculated vessels to 200 kPa with N2/CO2/O2.
       
  *Marine medium/synthetic seawater mix solution
   NaCl47g
   MgCl2·6H2O18g
   MgSO4·7H2O7g
   CaCl2·2H2O3g
   Na2SO43.13g
   Na2CO30.1g
   NaBr0.2g
   SrCl2·6H2O72mg
   H3BO352mg
   Na2HPO48.1mg
   NaF2.4mg
   KI0.05mg
   Sodium silicate0.4g
   Distilled water1L
       
  **Trace elements
   Nitrilotriacetic acid1.5g
   MgSO4·7H2O3g
   MnSO4·2H2O0.5g
   NaCl1g
   FeSO4·7H2O0.1g
   CoSO4·7H2O0.18g
   CaCl2·2H2O0.1g
   ZnSO4·7H2O0.18g
   CuSO4·5H2O0.01g
   H3BO30.01g
   Na2MoO4·2H2O0.01g
   NiCl2·6H2O0.025g
   Na2SeO3·5H2O0.4mg
   Na2WO40.4mg
   KAl(SO4)2·12H2O2mg
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
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