Search Results Detail

Medium Detail Information

Medium No.
Pyrobaculum aerophilum Medium
 Marine medium/synthetic seawater mix solution*125ml
 Trace elements**10ml
 Bacto Yeast Extract (Difco)0.5g
 Distilled water865ml
  After flushing with N2/CO2, adjust pH to 7.0 with 1 N H2SO4. Sterilize by filtration. Dispense the medium into vessels under a N2/CO2/O2 (79/20/1) atmosphere. Pressurize the inoculated vessels to 200 kPa with N2/CO2/O2.
  *Marine medium/synthetic seawater mix solution
   Sodium silicate0.4g
   Distilled water1L
  **Trace elements
   Nitrilotriacetic acid1.5g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).