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Medium Detail Information

Medium No.
Green Sulfur Bacterium Medium
 Ammonium acetate0.5g
 Na2S2O3 (if needed)0.5g
 Ferric citrate solution (0.1%)*5ml
 Vitamin B12 solution (0.001%)2ml
 Trace element solution SL-6**2ml
 Acetate solution***10ml
 Na2S·9H2O (neutralized)****0.6g
 Distilled water1L
  Mix ingredients except vitamin B12, acetate solution, NaHCO3 and Na2S·9H2O, distribute the medium into culture vessels, seal with butyl rubber stoppers, and autoclave (121°C, 15 min) under a N2/CO2 atmosphere (80/20). Aseptically add vitamin B12, acetate, NaHCO3 (30 g/L) and neutralized Na2S·9H2O solutions.
  *Ferric citrate
  Soluble not water.
  **Trace element solution SL-6
   Distilled water1L
  ***Acetate solution
   Ammonium acetate25g
   Magnesium acetate25g
   Distilled water1L
  Mix ingredients, adjust pH to 7.2, and autoclave under a N2 atmosphere.
  ****Na2S·9H2O (neutralized)
   Distilled water1L
  The sulfide solution is prepared in a vessel sealed with a butyl rubber stopper. Autoclave at 121℃ for 15 min under a N2 atmosphere. After cooling to room temperature, the pH is adjusted to about 7.3 by adding of sterile 2 N H2SO4 drop-wise with a syringe without opening the bottle. Appearance of yellow colour indicates the pH is about 8. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow.