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Potato Dextrose Agar (PDA)
Wash potatoes with tap water, peel and cut into 1 cm cubes. Rinse with tap water quickly and boil 200 g of potato cubes with 1 L of distilled water for 20 min. Mash and squeeze through a muslin bag. Add agar and boil till melted. Add glucose and stir till dissolved. Make up to 1 L. Adjust pH to 5.4 - 5.6. Autoclave to sterilize.
Commercial Potato dextrose agar (Difco, Oxoid or Nissui) is also available.
National Institute of Technology and Evaluation (JCN 9011005001123) 2-49-10 Nishihara, Shibuya-ku, Tokyo, Japan
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