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Medium Detail Information

Medium No.
859
Medium
Methanopyrus Medium
Composition
 NH4Cl0.25g
 K2HPO40.05g
 KH2PO40.09g
 NaCl11.8g
 MgSO4·7H2O1.75g
 MgCl2·6H2O4.5g
 Na2SO40.81g
 CaCl2·2H2O0.78g
 KCl0.3g
 NaHCO31g
 Fe(NH4)2(SO4)2·6H2O2mg
 (NH4)2Ni(SO4)2·6H2O2mg
 NaBr0.04g
 SrCl2·6H2O0.018g
 H3BO30.013g
 KI0.0125mg
 Sodium silicate1mg
 NaF0.6mg
 KNO30.4mg
 Na2HPO4·12H2O4.6mg
 Bacto Yeast Extract (Difco)0.2g
 Coenzyme M0.14g
 Trace elements solution*10ml
 Vitamin solution**10ml
 Resazurin1mg
 Na2S·9H2O0.5g
 Distilled water1L
  Mix ingredients except Na2S·9H2O, and adjust pH to 6.0 with H2SO4. Sterilize by filtration while gassing H2/CO2 (80/20). Separately autoclave Na2S·9H2O (5% solution) under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add Na2S·9H2O solution. Pressurize the inoculated vessels to 150 kPa with H2/CO2 (80/20).
       
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   KAl(SO4)2·12H2O0.02g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
       
  **Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
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