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Medium Detail Information

Medium No.
Methanopyrus Medium
 Sodium silicate1mg
 Bacto Yeast Extract (Difco)0.2g
 Coenzyme M0.14g
 Trace elements solution*10ml
 Vitamin solution**10ml
 Distilled water1L
  Mix ingredients except Na2S·9H2O, and adjust pH to 6.0 with H2SO4. Sterilize by filtration while gassing H2/CO2 (80/20). Separately autoclave Na2S·9H2O (5% solution) under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add Na2S·9H2O solution. Pressurize the inoculated vessels to 150 kPa with H2/CO2 (80/20).
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L