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Medium Detail Information

Medium No.
882
Medium
Deferribacter Medium
Composition
 NH4Cl0.535g
 KH2PO40.136g
 MgCl2·6H2O0.204g
 CaCl2·2H2O0.147g
 Trace elements solution*1ml
 Vitamin solution**1ml
 Sodium acetate0.82g
 Sodium nitrate0.85g
 NaHCO32.52g
 Resazurin1mg
 Na2S·9H2O0.5g
 Distilled water1L
  Mix ingredients except for vitamin solution and Na2S·9H2O, and autoclave under H2/CO2 (80/20) condition. Separately autoclave Na2S·9H2O as a 5% solution under a N2 atmosphere. Aseptically and anaerobically add filter-sterile vitamin solution and Na2S·9H2O solution, and pressurize inoculated bottles to 150 kPa with H2/CO2.
       
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
       
  **Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
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