Search Results Detail

Medium Detail Information

Medium No.
Deferribacter Medium
 Trace elements solution*1ml
 Vitamin solution**1ml
 Sodium acetate0.82g
 Sodium nitrate0.85g
 Distilled water1L
  Mix ingredients except for vitamin solution and Na2S·9H2O, and autoclave under H2/CO2 (80/20) condition. Separately autoclave Na2S·9H2O as a 5% solution under a N2 atmosphere. Aseptically and anaerobically add filter-sterile vitamin solution and Na2S·9H2O solution, and pressurize inoculated bottles to 150 kPa with H2/CO2.
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L