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Medium Detail Information

Medium No.
GS medium
 Trace mineral solution*10ml
 Trace vitamins**10ml
 Bacto Yeast Extract (Difco)2g
 Trypticase Peptone (BBL)2g
 Distilled water1L
 pH 7.0 
  Mix ingredients except for trace vitamins and Na2S, cysteine and autoclave under N2/CO2 (80/20) condition. Concentrated Na2S and cysteine solutions are sterilized separately by autoclaving under a N2 atmosphere in tightly closed vessels. Aseptically and anaerobically add filter-sterile trace vitamins, Na2S and cysteine solutions.
  *Trace mineral solution
   Nitrilotriacetic acid1.5g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add other minerals. Final pH 7.0 (with KOH). Sterilize by filtration.
  **Trace vitamins
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L