| KH2PO4 | 0.75 | g | | K2HPO4 | 0.78 | g | | MgCl2·6H2O | 0.36 | g | | CaCl2·2H2O | 0.1 | g | | NH4Cl | 0.54 | g | | NaHCO3 | 5 | g | | Trace elements solution* | 10 | ml | | Vitamin solution** | 10 | ml | | Yeast extract | 2 | g | | Sucrose | 6.8 | g | | NaNO3 | 1.7 | g | | NaCl | 20 | g | | Distilled water | 1 | L | | pH unadjusted | | | | | Mix ingredients except sucrose, NaHCO3 and vitamin solution. Dispense the medium into suitable culture vessels under a stream of N2 and seal with butyl rubber stoppers. After autoclaving, add filter-sterile sucrose, NaHCO3 and vitamin solutions. | | | | | | | | | | *Trace elements solution | | | | 2Na-EDTA | 0.64 | g | | | | MgSO4·7H2O | 6.2 | g | | | | MnSO4·4H2O | 0.55 | g | | | | NaCl | 1 | g | | | | FeSO4·7H2O | 0.1 | g | | | | CoCl2·6H2O | 0.17 | g | | | | CaCl2·2H2O | 0.13 | g | | | | ZnSO4·7H2O | 0.18 | g | | | | CuSO4 | 0.05 | g | | | | KAl(SO4)2·12H2O | 0.018 | g | | | | H3BO3 | 0.01 | g | | | | Na2MoO4·2H2O | 0.011 | g | | | | NiCl2·6H2O | 0.025 | g | | | Dissolve the 2Na-EDTA in 500 ml of distilled water, and after adding the salts, make up to 1 L. | | | | | | | | | | **Vitamin solution | | | | Biotin | 2 | mg | | | | Folic acid | 2 | mg | | | | Pyridoxine-HCl | 10 | mg | | | | Thiamine-HCl | 5 | mg | | | | Riboflavin | 5 | mg | | | | Nicotinic acid | 5 | mg | | | | Ca-pantothenate | 5 | mg | | | | p-Aminobenzoic acid | 1 | mg | | | | Vitamin B12 | 0.01 | mg | | | | Distilled water | 1 | L |
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