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Medium Detail Information

Medium No.
Methanosaeta harundinacea medium
 Sodium acetate1.7g
 Trace elements solution*10ml
 Vitamin solution**10ml
 Bacto Yeast Extract (Difco)0.5g
 Hipolypepton (Nihon seiyaku)0.5g
 Distilled water1L
  Mix ingredients except for vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under N2/CO2 (80/20) condition. Separately autoclave cysteine-HCl and Na2S·9H2O as a 3% solution under a N2 atmosphere. Aseptically and anaerobically add filter-sterile vitamin, cysteine-HCl and Na2S·9H2O solutions. Final pH is 7.2-7.5.
  *Trace elements solution
   Nitrilotriacetic acid (NTA)4.1g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add other minerals. Final pH 7.0.
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L