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Medium Detail Information

Medium No.
MPA Medium
 Yeast extract0.5g
 Crotonic acid0.86g
 Trace element solution*1ml
 Vitamin solution**1ml
 Distilled water1L
  Mix ingredients except NaHCO3, cysteine-HCl, Na2S·9H2O and vitamin solution, and autoclave under a N2 atmosphere. Separately autoclave cysteine-HCl and Na2S·9H2O as 5% solutions under a N2 atmosphere. After adding filter-sterile NaHCO3 solution to the medium, adjust pH to 7.0. Prior to inoculation, aseptically and anaerobically add filter-sterile vitamin, cysteine-HCl and Na2S·9H2O solutions.
  *Trace element solution
   Distilled water1L
  **Vitamin solution
   p-Aminobenzoic acid5mg
   Nicotinic acid5mg
   Lipoic acid5mg
   Folic acid2mg
   Vitamin B120.1mg
   Distilled water1L