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Medium No.
Sulfolobus metallicus medium
 Bacto Yeast Extract (Difco)1g
 Sulfur (powder)1g
 Distilled water1L
 pH 2.5 
  Mix ingredients except Bacto Yeast Extract and sulfur, adjust pH to 2.5 with H2SO4, and autoclave at 121°C for 15 min. Separately autoclave Bacto Yeast Extract as a 10% solution. Sulfur is sterilized separately by autoclaving for 60 min at 105°C on each of 3 successive days. Prior to inoculation, add Bacto Yeast Extract solution and sulfur powder. Cultivate under air containing 1-10% CO2.