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Medium No.	932
Medium	Sulfolobus metallicus medium
Composition
(NH4)2SO4 1.3 g   KH2PO4 0.28 g   MgSO4·7H2O 0.25 g   CaCl2·2H2O 0.07 g   FeCl3·6H2O 0.02 g   MnCl2·4H2O 1.8 mg   Na2B4O7·10H2O 4.5 mg   ZnSO4·7H2O 0.22 mg   CuCl2·2H2O 0.05 mg   Na2MoO4·2H2O 0.03 mg   VOSO4·nH2O 0.03 mg   CoSO4·7H2O 0.01 mg   Bacto Yeast Extract (Difco) 1 g   Sulfur (powder) 1 g   Distilled water 1 L   pH 2.5       Mix ingredients except Bacto Yeast Extract and sulfur, adjust pH to 2.5 with H2SO4, and autoclave at 121°C for 15 min. Separately autoclave Bacto Yeast Extract as a 10% solution. Sulfur is sterilized separately by autoclaving for 60 min at 105°C on each of 3 successive days. Prior to inoculation, add Bacto Yeast Extract solution and sulfur powder. Cultivate under air containing 1-10% CO2.
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