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Medium Detail Information

Medium No.	935
Medium	Alkaline halophile medium
Composition
KH2PO4 1 g   KCl 1 g   NH4Cl 1 g   MgSO4·7H2O 0.24 g   CaSO4·2H2O 0.17 g   NaCl 200 g   Sodium glutamate 1 g   Bacto Yeast Extract (Difco) 5 g   Casamino acids 5 g   Trace elements solution* 1 ml   Na2CO3 5 g   Agar (if necessary) 20 g   Distilled water 1 L       Mix ingredients except Na2CO3 and adjust pH to 6.5 before autoclaving. Add Na2CO3 as a filter-sterile 10% solution after autoclaving. Check final pH to be 9.0-9.5. If agar medium is prepared, dissolve agar by heating before adding NaCl.                   *Trace elements solution       2Na-EDTA 0.64 g       MgSO4·7H2O 6.2 g       MnSO4·4H2O 0.55 g       NaCl 1 g       FeSO4·7H2O 0.1 g       CoCl2·6H2O 0.17 g       CaCl2·2H2O 0.13 g       ZnSO4·7H2O 0.18 g       CuSO4 0.05 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.011 g       NiCl2·6H2O 0.025 g     Dissolve the 2Na-EDTA in 500 ml of distilled water, and after adding the salts, make up to 1 L.
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