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Medium Detail Information

Medium No.
Modified Prosthecomicrobium and Ancalomicrobium Medium
 Bacto Peptone (Difco)0.15g
 Yeast extract0.15g
 Vitamin solution*10ml
 Modified Hutner's solution**20ml
 Agar (if needed)15g
 Distilled water970ml
 pH unadjusted 
  *Vitamin solution
   Folic acid2mg
   Vitamin B120.1mg
   Distilled water1L
  **Modified Hutner's solution
   Nitrilotriacetic acid (NTA)10g
   Metal salt solution 44***50ml
  Adjust volume to 1 L with distilled water.
  Dissolve and neutralize the nitrilotriacetic acid with KOH (7.3 g); add the other ingredients and adjust the pH to 6.6-6.8 before bringing the volume to 1.0 L with distilled water.
  ***Metal salt solution 44
   Distilled water100ml
  Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. Store in the dark and cold (5℃).