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Medium No.
943
Medium
Modified Prosthecomicrobium and Ancalomicrobium Medium
Composition
 Bacto Peptone (Difco)0.15g
 Yeast extract0.15g
 (NH4)2SO40.25g
 Glucose1g
 Vitamin solution*10ml
 Modified Hutner's solution**20ml
 Agar (if needed)15g
 Distilled water970ml
 pH unadjusted 
       
  *Vitamin solution
   Biotin2mg
   Folic acid2mg
   Thiamine-HCl5mg
   Ca-pantothenate5mg
   Vitamin B120.1mg
   Riboflavin5mg
   Distilled water1L
       
  **Modified Hutner's solution
   Nitrilotriacetic acid (NTA)10g
   MgSO4·7H2O29.7g
   CaCl2·2H2O3.34g
   (NH4)6Mo7O24·2H2O9.25mg
   FeSO4·7H2O99mg
   Metal salt solution 44***50ml
  Adjust volume to 1 L with distilled water.
  Dissolve and neutralize the nitrilotriacetic acid with KOH (7.3 g); add the other ingredients and adjust the pH to 6.6-6.8 before bringing the volume to 1.0 L with distilled water.
       
  ***Metal salt solution 44
   Na-EDTA250mg
   ZnSO4·7H2O1095mg
   FeSO4·7H2O0.5g
   MnSO4·H2O154mg
   CuSO4·5H2O39.2mg
   Co(NO3)2·6H2O24.8mg
   Na2B4O7·10H2O17.7mg
   Distilled water100ml
  Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. Store in the dark and cold (5℃).
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