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Medium Detail Information

Medium No.
 Trace element solution*10ml
 Vitamin solution**10ml
 Sodium acetate1g
 Bacto Yeast Extract (Difco)2g
 Distilled water1L
  Mix ingredients except vitamin solution, Na2S·9H2O and cysteine-HCl. Dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20), seal with butyl rubber stoppers, and autoclave at 121°C for 20 min. Separately autoclave cysteine-HCl and Na2S·9H2O as 5% solutions under a N2 atmosphere. Prior to inoculation, aspetically and anaerobically add filter-sterile vitamin solution, cysteine-HCl solution and Na2S·9H2O solution.
  *Trace element solution
   Nitrilotriacetic acid1.5g
   Distilled water1L
  Dissolve nitrilotoriacetic acid and adjust pH to 6.5 with KOH. Then add minerals. Adjust final pH to 7.0.
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
  ***Wako Pure Chemical Ind., Ltd., Osaka, Japan.