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Medium Detail Information

Medium No.
M medium (EBR596)
 Yeast extract2g
 Vitamin solution*10ml
 Mineral solution**10ml
 Distilled water1L
 pH 6.5 
  Mix ingredients except NaHCO3, cysteine-HCl, Na2S·9H2O and vitamin solution, distribute the medium into culture vessels under a stream of nitrogen gas and seal with butyl rubber stoppers. After autoclaving (121°C, 15 min), aseptically inject other ingredients. The pH is adjusted to 6.5 by adding of sterile 0.6 N H2SO4 with a syringe without opening the vessel.
  *Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B121mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
  **Mineral solution
   Nitrilotriacetic acid (NTA)4.5g
   Distilled water1L