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Medium Detail Information

Medium No.
974
Medium
Medium for strain RASEN
Composition
 Bacto Yeast Extract (Difco)1g
 NaCl30g
 K2HPO490mg
 KH2PO470mg
 CaCl2·2H2O1.06g
 MgCl2·6H2O4.18g
 KCl0.33g
 NH4Cl0.535g
 NaHCO32g
 Na2S·9H2O0.3g
 Cysteine-HCl0.3g
 Trace element solution*10ml
 Vitamin solution**10ml
 Resazurin1mg
 Distilled water1L
 pH 7 
  For vial cultures, dissolve all ingredients except for yeast extract, vitamin solution, Na2S·9H2O and cysteine-HCl (up to 900 ml), flush N2/CO2 (80/20) (pH 7), dispense 19 ml into 50 ml vials, seal with butyl rubber stoppers with flushing N2/CO2 (80/20) and autoclave. Add 0.5 ml of each of filter-sterilized Na2S·9H2O solution (0.3 g Na2S·9H2O/50 ml) and cysteine-HCl solution (0.3 g cysteine-HCl/50 ml) per each vial separately. Add 0.5 ml of filter-sterilized 40 g/l yeast extract solution. Inoculum size 5%. Growth was observed after 2 days of incubation.
       
  *Trace element solution
   Nitrilotriacetic acid0.15g
   MnCl2·4H2O0.53g
   CoCl2·6H2O0.42g
   ZnCl290mg
   CuCl2·2H2O7mg
   AlCl36mg
   H3BO310mg
   Na2MoO4·2H2O1mg
   SrCl2·6H2O10mg
   NaBr10mg
   KI10mg
   FeCl2·4H2O0.1g
   NiCl2·6H2O25mg
   Na2SeO30.2mg
   Na2WO4·2H2O0.3mg
   Distilled water1L
       
  **Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
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