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Medium Detail Information

Medium No.
Medium for strain RASEN
 Bacto Yeast Extract (Difco)1g
 Trace element solution*10ml
 Vitamin solution**10ml
 Distilled water1L
 pH 7 
  For vial cultures, dissolve all ingredients except for yeast extract, vitamin solution, Na2S·9H2O and cysteine-HCl (up to 900 ml), flush N2/CO2 (80/20) (pH 7), dispense 19 ml into 50 ml vials, seal with butyl rubber stoppers with flushing N2/CO2 (80/20) and autoclave. Add 0.5 ml of each of filter-sterilized Na2S·9H2O solution (0.3 g Na2S·9H2O/50 ml) and cysteine-HCl solution (0.3 g cysteine-HCl/50 ml) per each vial separately. Add 0.5 ml of filter-sterilized 40 g/l yeast extract solution. Inoculum size 5%. Growth was observed after 2 days of incubation.
  *Trace element solution
   Nitrilotriacetic acid0.15g
   Distilled water1L
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L