Search Results Detail

Medium Detail Information

Medium No.
TYL medium
 Trypticase Peptone (BBL)2g
 Bacto Yeast Extract (Difco)2g
 50% Sodium lactate25ml
 Biotin solution*2ml
 K solution**10ml
 M solution***2.5ml
 C solution****2.5ml
 15% Na2CO3 solution20ml
 Distilled water1L
 pH 7 
  Mix ingredients except for Na2CO3 and cysteine-HCl, dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20), seal with butyl rubber stoppers, and autoclave at 115℃ for 25 min. Aseptically and anaerobically add filter-sterile Na2CO3 and cysteine-HCl solutions, and adjust pH to 7.0 with 3 N HCl.
  *Biotin solution
  **K solution
   Distilled water1L
  ***M solution
   Distilled water1L
  ****C solution
   Distilled water1L