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Medium Detail Information

Medium No.	989
Medium	TYL medium
Composition
Trypticase Peptone (BBL) 2 g   Bacto Yeast Extract (Difco) 2 g   50% Sodium lactate 25 ml   Biotin solution* 2 ml   K solution** 10 ml   M solution*** 2.5 ml   C solution**** 2.5 ml   Resazurin 1 mg   15% Na2CO3 solution 20 ml   Cysteine-HCl 0.75 g   Distilled water 1 L   pH 7       Mix ingredients except for Na2CO3 and cysteine-HCl, dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20), seal with butyl rubber stoppers, and autoclave at 115℃ for 25 min. Aseptically and anaerobically add filter-sterile Na2CO3 and cysteine-HCl solutions, and adjust pH to 7.0 with 3 N HCl.                   *Biotin solution       Biotin 5 mg       Ethanol 100 ml                   **K solution       KH2PO4 90 g       NaCl 90 g       (NH4)2SO4 90 g       Distilled water 1 L                   ***M solution       MnCl2·4H2O 4 g       CoCl2·6H2O 4 g       Distilled water 1 L                   ****C solution       CaCl2·2H2O 10.6 g       Distilled water 1 L
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