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Medium Detail Information

Medium No.
MMJS medium
 Bacto Yeast Extract (Difco)1g
 Trace elements solution*10ml
 Vitamin solution**2ml
 Sulfur (powder)30g
 Distilled water1L
  Mix ingredients except for vitamin solution, sulfur, NaHCO3, and Na2S·9H2O. Dispense the medium into suitable culture vessels under a stream of H2/CO2 (80/20), seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Separately autoclave Na2S·9H2O (5% solution) under N2 atmosphere. Sterilize vitamin and NaHCO3 solutions by filtration. Sulfur is sterilized separately by autoclaving for 60 min at 105°C on each of 3 successive days. Prior to inoculation, aseptically and anaerobically add vitamin, sulfur, NaHCO3, and Na2S·9H2O, and pressurize the inoculated vessels to 150 kPa with H2/CO2 (80/20). Final pH is 5.5-6.0.
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH is 7.0.
  **Vitamin solution
   Folic acid2mg
   Nicotinic acid5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L