遺伝子 : Salicylate 5-hydroxylase alpha subunit (NAGG)

遺伝子情報

ID	NRULE_0191
遺伝子ID	NAGG
作成日	2013/04/09
タンパク質名	Protein name Full Salicylate 5-hydroxylase alpha subunit EC EC 1.14.13.- Synonym Full Salicylate 5-monooxygenase large subunit
遺伝子名	nagG
遺伝子条件	BLASTP; O52379; hit=1; score=900; pid=42
参照	Gene Ontology GO GO:0051537, F:2 iron, 2 sulfur cluster binding, IEA:UniProtKB-KW GO:0005506, F:iron ion binding, IEA:InterPro GO:0016708, F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of two atoms of oxygen intoone donor, IEA:InterPro GO:0019439, P:aromatic compound catabolic process, IEA:InterPro Enzyme and pathway databases UniPathway UPA00082, - Family and domain databases InterPro IPR017941, Rieske_2Fe-2S IPR015881, Ring-hydroxy_dOase_2Fe2S_BS IPR015879, Ring_hydroxy_dOase_asu_C_dom IPR001663, Rng_hydr_dOase-A Pfam PF00355, Rieske, 1 PF00848, Ring_hydroxyl_A, 1 PRINTS PR00090, RNGDIOXGNASE PROSITE PS51296, RIESKE, 1 PS00570, RING_HYDROXYL_ALPHA, 1
配列の特徴	O52379 Ralstonia sp. METAL 91 Iron-sulfur (2Fe-2S). METAL 93 Iron-sulfur (2Fe-2S); via pros nitrogen METAL 111 Iron-sulfur (2Fe-2S). METAL 114 Iron-sulfur (2Fe-2S). METAL 224 Iron. METAL 229 Iron. METAL 370 Iron.
注釈	Function Hydroxylase component of the salicylate 5-hydroxylase multicomponent enzyme system which catalyzes the 5-hydroxylation of salicylate to gentisate Catalytic activity Salicylate + NADH + O(2) = 2,5-dihydroxybenzoate + NAD(+) + H(2)O. Cofactor Binds 1 iron ion per subunit Binds 1 [2Fe-2S] cluster per subunit Pathway Aromatic compound metabolism; naphthalene degradation Subunit structure The salicylate 5-hydroxylase multicomponent enzyme system is composed a hydroxylase component (NagG and NagH) and an electron transfer component (ferredoxin and ferredoxin reductase). Sequence similarities Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family Contains 1 Rieske domain
参考文献	O52379 Ralstonia sp. 9573207 A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2. (J Bacteriol. 1998) 11872705 Salicylate 5-hydroxylase from Ralstonia sp. strain U2: a monooxygenase with close relationships to and shared electron transport proteins with naphthalene dioxygenase. (J Bacteriol. 2002)

関与する機能

機能名
Naphthalene degradation (via hydroxylation by dioxygenase)

該当CDS

学名	NBRC番号	CDS	タンパク質名
Marinomonas mediterranea	103028	Marme_2404	Biphenyl 2,3-dioxygenase
Shimwellia blattae	105725	EB105725_26_00440	3-phenylpropionate/cinnamic acid dioxygenase alpha subunit
Sphingomonas wittichii	105917	Swit_3056	Rieske (2Fe-2S) domain protein