遺伝子 : D-alanine--D-serine ligase (VANG)

遺伝子情報

ID	NRULE_0271
遺伝子ID	VANG
作成日	2014/06/27
タンパク質名	Protein name Full D-alanine--D-serine ligase Short D-Ala--D-Ser ligase EC EC 6.3.2.-
遺伝子名	vanG
遺伝子条件	HAMAP; MF_00047; hit=1ANDBLASTP; Q6WRY5; hit=1; score=1500; pid=80
参照	Gene Ontology GO GO:0005737, C:cytoplasm, IEA:UniProtKB-SubCell GO:0005524, F:ATP binding, IEA:UniProtKB-HAMAP GO:0008716, F:D-alanine-D-alanine ligase activity, IEA:UniProtKB-HAMAP GO:0000287, F:magnesium ion binding, IEA:UniProtKB-HAMAP GO:0030145, F:manganese ion binding, IEA:UniProtKB-HAMAP GO:0009252, P:peptidoglycan biosynthetic process, IEA:UniProtKB-HAMAP GO:0008360, P:regulation of cell shape, IEA:UniProtKB-KW Enzyme and pathway databases BioCyc MetaCyc:MONOMER-15480, - UniPathway UPA00219, - Family and domain databases HAMAP MF_00047, Dala_Dala_lig, 1 InterPro IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR013816, ATP_grasp_subdomain_2 IPR000291, D-Ala_lig_Van_CS IPR005905, D_ala_D_ala IPR011095, Dala_Dala_lig_C IPR011127, Dala_Dala_lig_N IPR016185, PreATP-grasp_dom Pfam PF07478, Dala_Dala_lig_C, 1 PF01820, Dala_Dala_lig_N, 1 TIGRFAMs TIGR01205, D_ala_D_alaTIGR, 1 PROSITE PS50975, ATP_GRASP, 1 PS00843, DALA_DALA_LIGASE_1, 1 PS00844, DALA_DALA_LIGASE_2, 1
配列の特徴	Q6WRY5 Enterococcus faecalis (Streptococcus faecalis). NP_BIND 173 - 228 ATP (By similarity). NP_BIND 181 - 183 ADP. NP_BIND 219 - 222 ADP. NP_BIND 311 - 312 ADP. METAL 299 Magnesium or manganese 1 (By similarity). METAL 312 Magnesium or manganese 1 (By similarity). METAL 312 Magnesium or manganese 2 (By similarity). METAL 314 Magnesium or manganese 2 (By similarity). BINDING 140 ADP. BINDING 190 ADP. BINDING 212 Sulfate 2. BINDING 223 Sulfate 3. BINDING 226 ADP. BINDING 261 ADP. BINDING 270 Sulfate 4. BINDING 297 Sulfate 5. BINDING 297 Sulfate 6. BINDING 301 ADP. BINDING 314 Sulfate 5. BINDING 318 Sulfate 5; via amide nitrogen. BINDING 318 Sulfate 6; via amide nitrogen. BINDING 324 Sulfate 6; via amide nitrogen. BINDING 336 Sulfate 7; via amide nitrogen. BINDING 336 Sulfate 8; via amide nitrogen.
注釈	Function Cell wall formation (By similarity) Catalytic activity ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine Cofactor Binds 2 magnesium or manganese ions per subunit (By similarity) Pathway Cell wall biogenesis; peptidoglycan biosynthesis Sequence similarities Belongs to the D-alanine--D-alanine ligase family Contains 1 ATP-grasp domain Contains ATP-grasp domain
参考文献	Q6WRY5 Enterococcus faecalis (Streptococcus faecalis). 19851013 Crystallization and preliminary X-ray analysis of a D-Ala:D-Ser ligase associated with VanG-type vancomycin resistance. (Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009) 22969085 Structural and functional characterization of VanG D-Ala:D-Ser ligase associated with vancomycin resistance in Enterococcus faecalis. (J Biol Chem. 2012)

関与する機能

機能名
Vancomycin resistance (via D-Ala-D-Ser precursor)

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