Amph_00010 : CDS information

Location

Organism	Streptomyces nodosus
Strain	ATCC 14899 (=NBRC 12895)
Entry name	Amphotericin B
Contig	AF357202
Start / Stop / Direction	1,824 / 4 / - [in whole cluster] 1,824 / 4 / - [in contig]
Location	complement(4..1824) [in whole cluster] complement(4..1824) [in contig]
Type	CDS
Length	1,821 bp (606 aa)

Annotation

Category	4.3 transport
Product	putative ABC transporter ATP-binding/permease protein
Product (GenBank)	AmphG
Gene
Gene (GenBank)	amphG
EC number
Keyword
Note
Note (GenBank)	possible amphotericin export protein
Reference	ACC Q93NY2 PmId [11451671] Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes. (Chem Biol. , 2001) comment Amphotericin生合成gene clusterの報告。 amphG: ABC transporter AmphG and AmphHはheterodimerを形成してAmphotericinを排出する可能性あり。またはひとつがself-resistanceの役割をしうる。
Related Reference	ACC Q9L4X6 NITE Nysta_00020 PmId [16251298] Nystatin biosynthesis and transport: nysH and nysG genes encoding a putative ABC transporter system in Streptomyces noursei ATCC 11455 are required for efficient conversion of 10-deoxynystatin to nystatin. (Antimicrob Agents Chemother. , 2005) comment 2nd(Q9L4X6) 80%, 0.0 Streptomyces noursei_nysG [Nysta_00020]ABC transporter nysG, nysHを不活化してもnystatinは細胞外へ運ばれるので、他にも代替的なtransporterがあるようだ。不活化すると10-deoxynystatinの産生量が増えるので、C-10 hydroxylation(NysL)の効率に関係がある。ATPase inhibitor sodium o-vanadateを使った実験で産生量が減少する。domainについても検討し、明らかにtype III ABC transportersに属するといっている。

Sequence

>putative ABC transporter ATP-binding/permease protein [AmphG]
MAPDVPEEHEEERESEQPVRRLAALLRPHRRSVGLALTAGVVGILLNAFGPLLLGRVTDL
IADGVLGHGGPAPGVDFGALGRLLMILLVLYVVASVFMLVQNWLVASVVRLLIHDLRHRA
QEKLARLPLRYFDRKPAGETLSRGTDDVDNLQQTLQQTLTDLISSVFSLVIMLSLMLIIS
PSLAGVMLLSVPVSGLLAAWISKRAQPQYAAQWSASGKLTAHVEEMCAGHALVKAFDRRA
EAEQRFDERNEAVYRAGSGAQFASGAIEPVMMFVANLGYVAVAVVGAWKVVNGSLTLGDV
QAFILYARQFSQPIVEIASVAGRLQSGVASAQRVFTLLDAPEQEPEPDRPLAVERVEGRV
EFQDVSFRYSPDTPLIEGLSLSVEPGSTVAVVGPSGAGKTTVANLLMRFYEIDSGRILLD
GTDTAAMNRDDLRSRFGLVLQDTWLFKGTIAENIAYGSPGATRADIVEAARATYADRFIR
TLSQGYDTVLDDESGGVSAGEKQLITVARAFLARPAVLVLDEATSSVDTRTELLIQRAMN
TLRPGRTSFVIAHRLSTIRDADVIVVMKSGRIVEQGTHDQLIDAQGAYARLHAARADAPA
ADVTVG

>putative ABC transporter ATP-binding/permease protein [AmphG]
ATGGCGCCGGACGTTCCTGAGGAGCACGAAGAGGAGCGGGAGTCGGAGCAGCCGGTCCGC
CGGCTCGCCGCTCTGCTGCGCCCGCACCGCCGGTCGGTGGGCCTCGCCCTCACGGCGGGT
GTCGTCGGCATCCTCCTCAACGCCTTCGGCCCGCTGCTGCTGGGCCGGGTCACCGACCTG
ATCGCCGACGGCGTCCTCGGGCACGGCGGACCGGCCCCGGGCGTCGACTTCGGGGCGCTC
GGCAGACTGCTGATGATCCTGCTGGTGCTGTATGTGGTGGCATCGGTGTTCATGCTGGTC
CAGAACTGGCTGGTGGCCTCGGTGGTCCGCCTGCTCATCCACGACCTGCGGCACCGGGCG
CAGGAGAAGCTGGCGCGGCTGCCGCTGCGCTACTTCGACCGGAAGCCGGCGGGCGAGACG
CTCAGCCGCGGCACGGACGACGTCGACAACCTCCAGCAGACCCTCCAGCAGACCCTGACC
GATCTGATCAGCTCGGTGTTCTCGCTGGTCATCATGTTGTCCCTGATGCTGATCATCTCG
CCCTCGCTGGCCGGGGTGATGCTGCTGAGCGTCCCGGTGTCGGGGCTGCTCGCCGCCTGG
ATCAGCAAGCGGGCCCAGCCGCAGTACGCGGCCCAGTGGTCCGCGAGCGGCAAGCTGACC
GCGCATGTCGAGGAGATGTGCGCCGGGCACGCGCTGGTCAAGGCCTTCGACCGGCGGGCG
GAGGCCGAGCAGCGCTTCGACGAGCGCAACGAGGCGGTGTACCGGGCCGGTTCGGGGGCG
CAGTTCGCGTCCGGCGCGATCGAGCCCGTGATGATGTTCGTCGCCAACCTCGGCTATGTC
GCGGTCGCCGTCGTCGGCGCCTGGAAGGTCGTCAACGGCTCACTGACGCTGGGCGATGTG
CAGGCGTTCATCCTGTACGCACGGCAGTTCAGCCAGCCGATCGTGGAGATCGCCTCGGTC
GCGGGCCGCCTCCAGTCCGGGGTGGCCTCCGCGCAGCGGGTGTTCACGCTGCTGGACGCC
CCGGAGCAGGAACCCGAGCCGGACCGCCCGCTCGCGGTGGAACGCGTCGAGGGCCGGGTG
GAGTTCCAGGACGTGTCGTTCCGCTACTCCCCCGACACCCCGCTCATCGAGGGTCTTTCG
CTGTCCGTGGAACCCGGCAGCACGGTGGCGGTCGTCGGACCGAGCGGCGCCGGCAAGACC
ACGGTCGCCAATCTGCTGATGCGCTTCTACGAGATCGACTCGGGCCGCATCCTGCTGGAC
GGCACCGACACCGCCGCGATGAACCGCGACGACCTGCGGTCCCGCTTCGGCCTCGTCCTC
CAGGACACCTGGCTGTTCAAGGGCACGATCGCCGAGAACATCGCCTACGGGTCCCCGGGC
GCGACCCGCGCCGACATCGTGGAGGCGGCCCGCGCGACCTACGCCGACCGGTTCATCCGC
ACCCTGTCGCAGGGGTACGACACGGTCCTGGACGACGAGTCGGGCGGTGTCAGCGCCGGG
GAGAAGCAGCTGATCACGGTCGCGAGGGCGTTCCTCGCCCGGCCGGCCGTGCTCGTCCTG
GACGAGGCGACCAGCTCCGTGGACACCCGTACCGAGCTGCTGATCCAGCGGGCCATGAAT
ACCCTGCGGCCGGGCCGGACGAGCTTTGTGATCGCGCACCGTCTGTCCACCATCAGGGAC
GCCGATGTCATCGTCGTGATGAAGTCCGGCCGGATCGTCGAGCAGGGCACCCATGACCAG
CTCATCGACGCCCAGGGAGCCTACGCCCGGCTGCACGCCGCCCGCGCCGACGCGCCCGCC
GCCGATGTGACGGTCGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001140 ABC transporter, transmembrane domain (Domain) PF00664 ABC_membrane IPR003439 ABC transporter-like (Domain) PF00005 ABC_tran PS50893 ABC_TRANSPORTER_2 IPR003593 AAA+ ATPase domain (Domain) SM00382 AAA IPR011527 ABC transporter, transmembrane domain, type 1 (Domain) SSF90123 ABC_TM_1 IPR017871 ABC transporter, conserved site (Conserved_site) PS00211 ABC_TRANSPORTER_1 IPR017940 ABC transporter, integral membrane type 1 (Domain) PS50929 ABC_TM1F
SignalP	Eukaryota
TMHMM	Transmembrane 3

first Amph_00020