Amph_00010 Amph_00010   Amph_00030 Amph_00030

Amph_00020 : CDS information

close this sectionLocation

Organism
StrainATCC 14899 (=NBRC 12895)
Entry nameAmphotericin B
Contig
Start / Stop / Direction3,628 / 1,805 / - [in whole cluster]
3,628 / 1,805 / - [in contig]
Locationcomplement(1805..3628) [in whole cluster]
complement(1805..3628) [in contig]
TypeCDS
Length1,824 bp (607 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative ABC transporter ATP-binding/permease protein
Product (GenBank)AmphH
Gene
Gene (GenBank)amphH
EC number
Keyword
Note
Note (GenBank)
  • possible amphotericin export protein
Reference
ACC
PmId
[11451671] Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes. (Chem Biol. , 2001)
comment
Amphotericin生合成gene clusterの報告。
amphH: ABC transporter

AmphG and AmphHはheterodimerを形成してAmphotericinを排出する可能性あり。
またはひとつがself-resistanceの役割をしうる。
Related Reference
ACC
Q9L4X5
NITE
Nysta_00030
PmId
[16251298] Nystatin biosynthesis and transport: nysH and nysG genes encoding a putative ABC transporter system in Streptomyces noursei ATCC 11455 are required for efficient conversion of 10-deoxynystatin to nystatin. (Antimicrob Agents Chemother. , 2005)
comment
3rd(Q9L4X5) 76%, 0.0
Streptomyces noursei_nysH
[Nysta_00030]ABC transporter

nysG, nysHを不活化してもnystatinは細胞外へ運ばれるので、他にも代替的なtransporterがあるようだ。不活化すると10- deoxynystatinの産生量が増えるので、C-10 hydroxylation(NysL)の効率に関係がある。ATPase inhibitor sodium o-vanadateを使った実験で産生量が減少する。domainについても検討し、明らかにtype III ABC transportersに属するといっている。

close this sectionSequence

selected fasta
>putative ABC transporter ATP-binding/permease protein [AmphH]
MAPSVRWPTPFQRAAAGRRQPGCHSVLLRLMRTQLRPYAGSVVALVVLHLVQILGTLLLP
TLGAALIDEGVVRHDSDRIGTIGATMAVVALVQIAAALGAAALGARTSTALGRDLRSAVF
RRVLDFSAREIGRFGTPSLLTRSVNDVQQVQNLAQSGLGIFVAAPLMCLGSVLLALRQDV
TLALILVPMVLVVAVCFGLLLSRMAALYARLQQTLDRIGRLLRERITGVRVVRSFARDAH
EGERFTRTNEELLGLSLGVGRLIAVMLPSVLLLMNLFTLGLLWVGARRIDSGSMQIGALS
AFLSYLSLILMSVVMLAFVFLNVPRARVCAERITEVLQAETDVVPPASPRPMAGPAGQVE
LVGAEFRYPGAENAVLRDLSLTLRPGERVAVLGSTGSGKTTLLHLILRLVDVTAGEVRIG
GTDVRELDPSVMAAAVGYVPQRPYLFAGTVASNMRFGRPDATDEELWEVLRIAQHEGFVT
RLGGLDTEIAQGGTTVSGGQRQRLAIARALLRRPAIYLFDDSFSALDQSTEAALRKALVP
YTEGATVITVAQRVASVRDADRIVLLDQGGIAATGTHDALLRDSPTYREIALSQRTREET
AHGAGRS
selected fasta
>putative ABC transporter ATP-binding/permease protein [AmphH]
ATGGCCCCGTCGGTGCGCTGGCCGACTCCGTTCCAACGGGCGGCTGCGGGCCGCCGTCAA
CCGGGGTGTCATTCCGTGCTGTTGAGACTCATGCGCACACAACTGCGCCCCTATGCCGGG
TCCGTTGTCGCTCTGGTCGTTTTGCATCTGGTGCAGATCCTCGGCACGCTGCTGCTGCCG
ACGCTGGGCGCCGCACTCATCGACGAGGGAGTCGTCCGCCACGACAGCGACCGCATCGGC
ACCATCGGCGCCACGATGGCCGTGGTGGCGCTGGTGCAGATCGCCGCGGCGCTGGGTGCC
GCGGCGCTGGGCGCACGCACCTCCACCGCTCTCGGCCGGGATCTGCGGTCCGCGGTCTTC
CGCCGGGTGCTGGACTTCTCCGCCCGTGAGATCGGCCGGTTCGGCACTCCGTCCCTGCTG
ACCCGTTCGGTGAACGACGTCCAGCAGGTGCAGAACCTCGCCCAGTCCGGCCTCGGCATC
TTCGTGGCGGCTCCCCTGATGTGCCTGGGCAGTGTGCTGCTCGCGCTGCGCCAGGATGTG
ACGCTGGCCCTGATCCTGGTCCCGATGGTGCTGGTGGTGGCCGTCTGCTTCGGTCTGCTG
CTGTCCCGGATGGCCGCGCTGTACGCCCGGCTCCAGCAGACGCTGGACCGTATCGGGCGA
CTGCTGCGGGAGCGCATCACCGGGGTGCGTGTGGTGCGTTCCTTCGCCCGCGACGCCCAT
GAGGGCGAGCGCTTCACCCGCACCAACGAGGAACTCCTCGGCCTGTCCCTGGGGGTGGGC
CGGCTCATCGCGGTGATGCTGCCGTCGGTGCTGCTGCTGATGAACCTCTTCACCCTGGGG
CTGCTGTGGGTGGGGGCCCGCCGGATCGACTCGGGCAGCATGCAGATCGGTGCGCTCAGC
GCCTTCCTCAGCTATCTGTCGCTCATCCTGATGTCCGTGGTGATGCTCGCCTTCGTGTTC
CTGAACGTGCCGCGGGCCCGGGTGTGCGCGGAGCGGATCACGGAGGTCCTCCAGGCGGAG
ACCGATGTCGTCCCGCCCGCCTCGCCGCGGCCCATGGCGGGTCCCGCCGGGCAGGTCGAG
CTGGTGGGCGCCGAGTTCCGCTACCCAGGTGCCGAGAACGCCGTGCTGCGGGATCTGTCG
CTGACGCTGCGGCCCGGTGAGCGGGTCGCGGTCCTCGGCAGCACCGGCTCCGGCAAGACC
ACGCTGCTGCATCTGATCCTGCGGCTGGTCGACGTCACCGCGGGCGAGGTGCGGATCGGC
GGCACCGATGTGCGCGAACTGGACCCGTCGGTGATGGCCGCCGCCGTGGGCTATGTGCCG
CAGCGTCCGTATCTGTTCGCCGGGACCGTCGCGAGCAATATGCGCTTCGGCCGGCCGGAC
GCCACGGACGAGGAGCTGTGGGAGGTGCTGCGGATCGCCCAGCACGAAGGCTTCGTGACC
CGGCTCGGCGGCCTCGACACGGAGATCGCTCAGGGCGGCACCACCGTCTCCGGCGGTCAG
CGCCAGCGGCTGGCGATCGCACGGGCGCTGCTGCGCCGCCCCGCCATCTATCTCTTCGAC
GACTCCTTCTCGGCGCTCGACCAGAGCACGGAGGCGGCGCTACGGAAGGCCCTGGTGCCC
TACACCGAGGGCGCCACCGTGATCACCGTGGCGCAGCGCGTCGCCTCCGTGCGCGACGCC
GACCGGATCGTCCTCCTGGACCAGGGCGGCATCGCCGCCACCGGCACCCATGACGCGCTG
CTGCGCGACAGTCCCACCTACCGCGAGATCGCGCTCTCCCAGCGCACCCGAGAGGAAACC
GCTCATGGCGCCGGACGTTCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001140 ABC transporter, transmembrane domain (Domain)
[45-313] 3.90000000000001e-32 PF00664
PF00664   ABC_membrane
IPR003439 ABC transporter-like (Domain)
[359-593] PS50893
PS50893   ABC_TRANSPORTER_2
[400-522] 2.4e-19 PF00005
PF00005   ABC_tran
IPR003593 AAA+ ATPase domain (Domain)
[385-570] 3.00000067992871e-18 SM00382
SM00382   AAA
IPR011527 ABC transporter, transmembrane domain, type 1 (Domain)
[31-345] 6.89998448199219e-60 SSF90123
SSF90123   ABC_TM_1
IPR017871 ABC transporter, conserved site (Conserved_site)
[496-510] PS00211
PS00211   ABC_TRANSPORTER_1
IPR017940 ABC transporter, integral membrane type 1 (Domain)
[43-325] PS50929
PS50929   ABC_TM1F
SignalP
[1-1] Signal-anchor
Eukaryota   
TMHMM
[45-67] Transmembrane (i-o) [82-104] Transmembrane (o-i) [157-176] Transmembrane (i-o) [180-202] Transmembrane (o-i) [264-286] Transmembrane (i-o) [301-323] Transmembrane (o-i)
Transmembrane 6   
Amph_00010 Amph_00010   Amph_00030 Amph_00030
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