Amph_00030 : CDS information

Location

Organism	Streptomyces nodosus
Strain	ATCC 14899 (=NBRC 12895)
Entry name	Amphotericin B
Contig	AF357202
Start / Stop / Direction	3,840 / 4,874 / + [in whole cluster] 3,840 / 4,874 / + [in contig]
Location	3840..4874 [in whole cluster] 3840..4874 [in contig]
Type	CDS
Length	1,035 bp (344 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative GDP-mannose 4,6-dehydratase putative GDP-D-mannose dehydratase
Product (GenBank)	AmphDIII
Gene	amphDIII gmd
Gene (GenBank)	amphDIII
EC number	4.2.1.47
Keyword	mycosamine
Note
Note (GenBank)	mycosamine biosynthesis protein
Reference	ACC Q93NY0 PmId [11451671] Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes. (Chem Biol. , 2001) [14700629] Biosynthesis of deoxyamphotericins and deoxyamphoteronolides by engineered strains of Streptomyces nodosus. (Chem Biol. , 2003) comment [PMID: 11451671](2001) Amphotericin生合成gene clusterの報告。 amphDIII: GDP-mannose-4,6-DH -- [PMID:14700629](2003) mycosamine生合成に関連したGDP-D-mannose 4,6-dehydrataseをコードするamphDIII geneのmutantは、8-deoxyamphoteronolides A and Bを産生。よってmycosamineによるglycosylationはC-8 hydroxylationより前に、環外carboxyl基形成はこれら2つの修飾より前に起こる。
Related Reference	ACC Q9L4X4 NITE Nysta_00040 PmId [17905880] Analysis of the mycosamine biosynthesis and attachment genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455. (Appl Environ Microbiol. , 2007) comment 2nd(Q9L4X4) 89%, 0.0 Streptomyces noursei_nysDIII [Nysta_00040]GDP-mannose 4,6-dehydratase(EC 4.2.1.47) NysDIIIがGDP-mannose→GDP-4-keto-6-deoxy-D-mannoseへ変換することを反応産物のHPLCで確認している。 GDP-4-keto-6-deoxy-D-mannose→GDP-3-keto-6-deoxy-D-mannoseへの異性化は自発的であると提唱されている。

Sequence

>putative GDP-mannose 4,6-dehydratase [AmphDIII]
MPKRALITGITGQDGSYLAEHLLSQGYQVWGLIRGQANPRKFRVSRLASELSFVDGDLMD
QGSLVSAVDKVQPDEVYNLGAISFVPMSWQQAELVTEVNGMGVLRVLEAIRMVSGLSMSR
TAGTEGQIRFYQASSSEMFGKVAETPQRETTLFHPRSPYGAAKAYGHFITRNYRESFGMY
AVSGMLFNHESPRRGQEFVTRKISLAVARIKLGLQDKLALGNMDAVRDWGYAGDYVRAMH
LMLQQDAPDDYVIGTGEMHTVRDAVRFAFEHVGLDWKDYVVVDPDLVRPAEVEVLCADSS
KAQAQLGWKPSVDFQELMRMMVDADLASVSRQNELDDLLLAHSW

>putative GDP-mannose 4,6-dehydratase [AmphDIII]
ATGCCCAAGCGCGCACTGATCACCGGAATTACCGGCCAGGACGGGTCCTATCTGGCGGAG
CACCTGCTGTCGCAGGGGTACCAGGTGTGGGGTCTCATCCGTGGCCAGGCCAACCCACGC
AAGTTCCGGGTGAGCCGGCTCGCCTCCGAACTCTCCTTCGTCGACGGTGACCTCATGGAC
CAGGGCAGCCTGGTCTCGGCGGTCGACAAGGTACAGCCGGACGAGGTGTACAACCTCGGC
GCGATCTCGTTCGTGCCGATGTCCTGGCAGCAGGCCGAGCTGGTCACCGAGGTGAACGGC
ATGGGCGTGCTGCGGGTCCTGGAGGCCATCCGCATGGTGAGCGGACTGAGCATGTCCCGC
ACCGCGGGCACCGAGGGCCAGATCCGTTTCTACCAGGCGTCCTCCTCGGAGATGTTCGGC
AAGGTCGCCGAGACGCCGCAGCGGGAGACCACGCTCTTCCACCCGCGCAGCCCCTACGGC
GCGGCCAAGGCGTACGGTCACTTCATCACCCGCAACTACCGTGAGTCCTTCGGCATGTAC
GCGGTCTCCGGGATGCTGTTCAACCATGAATCGCCCCGCCGTGGCCAGGAGTTCGTGACC
CGCAAGATCTCCCTCGCGGTCGCCCGCATCAAGCTGGGGCTCCAGGACAAGCTGGCCCTC
GGCAACATGGACGCGGTCCGCGACTGGGGTTACGCGGGCGACTACGTCCGCGCCATGCAT
CTGATGCTCCAGCAGGACGCCCCCGACGACTATGTGATCGGCACCGGGGAGATGCACACC
GTGCGCGACGCGGTCCGCTTCGCCTTCGAGCACGTGGGCCTCGACTGGAAGGACTACGTC
GTCGTCGACCCCGACCTCGTGCGGCCCGCCGAGGTCGAGGTGCTGTGCGCCGACAGCTCC
AAGGCACAGGCCCAGCTGGGCTGGAAGCCGAGCGTCGATTTCCAGGAACTCATGCGGATG
ATGGTCGACGCCGACCTCGCGTCCGTTTCCCGGCAGAACGAACTCGACGACCTTCTGCTG
GCACACAGCTGGTAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Amph_00020 Amph_00040