Amph_00100 Amph_00100   Amph_00120 Amph_00120

Amph_00110 : CDS information

close this sectionLocation

Organism
StrainATCC 14899 (=NBRC 12895)
Entry nameAmphotericin B
Contig
Start / Stop / Direction61,995 / 61,798 / - [in whole cluster]
61,995 / 61,798 / - [in contig]
Locationcomplement(61798..61995) [in whole cluster]
complement(61798..61995) [in contig]
TypeCDS
Length198 bp (65 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.5 electron carrier
Productputative ferredoxin
Product (GenBank)AmphM
Gene
Gene (GenBank)amphM
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11451671] Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes. (Chem Biol. , 2001)
comment
Amphotericin生合成gene clusterの報告。
amphM: Ferredoxin
Related Reference
ACC
P18324
PmId
[1846297] Ferredoxins from two sulfonylurea herbicide monooxygenase systems in Streptomyces griseolus. (Biochemistry. , 1991)
comment
13th(P18324) 55%, 3e-09
Streptomyces griseolus_suaB
Ferredoxin-1
metal-ion binding site保存あり

sulfonylurea herbicideが誘発されたStreptomyces griseolusの可溶性タンパク質フラクションより、Fd-1 と Fd-2の二種類のフェレドキシンを精製し、どちらも、aerobic mixture of NADPH、spinach ferredoxin:NADP oxidoreductase、精製したcytochrome P-450SU1とherbicide基質にsulfonylurea monooxygenase活性を復元できることが示されている。また、cytochromes P-450, P-450SU1 (CYP105A1) と P-450SU2 (CYP105B1)は、それぞれFd-1 と Fd-2の近傍に位置していることが示されている。

close this sectionSequence

selected fasta
>putative ferredoxin [AmphM]
MVHVNVDVDRCVGAGQCVLTASDVFDQDDDGLVTVLAQPSDPSTVETVREAAVVCPSMAI
TVVEN
selected fasta
>putative ferredoxin [AmphM]
ATGGTGCACGTCAACGTGGACGTCGATCGCTGCGTCGGTGCCGGACAGTGCGTCCTGACC
GCGTCGGACGTGTTCGACCAGGACGACGACGGCCTGGTGACCGTCCTCGCACAGCCGTCG
GACCCGTCCACGGTGGAGACGGTCCGGGAGGCCGCGGTGGTCTGCCCCTCGATGGCGATC
ACCGTCGTGGAGAACTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001080 3Fe-4S ferredoxin (Family)
[6-17] 1.19999985703101e-05 PR00352 [17-27] 1.19999985703101e-05 PR00352 [48-60] 1.19999985703101e-05 PR00352
PR00352   3FE4SFRDOXIN
IPR010693 Ferredoxin soy-like (Family)
[2-65] 1.4e-14 PF06902
PF06902   DUF1271
SignalP No significant hit
TMHMM No significant hit
Amph_00100 Amph_00100   Amph_00120 Amph_00120
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