Amph_00110 Amph_00110   Amph_00130 Amph_00130

Amph_00120 : CDS information

close this sectionLocation

Organism
StrainATCC 14899 (=NBRC 12895)
Entry nameAmphotericin B
Contig
Start / Stop / Direction63,250 / 62,051 / - [in whole cluster]
63,250 / 62,051 / - [in contig]
Locationcomplement(62051..63250) [in whole cluster]
complement(62051..63250) [in contig]
TypeCDS
Length1,200 bp (399 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)AmphN
Gene
Gene (GenBank)amphN
EC number
Keyword
  • C-16 carboxy group formation
Note
Note (GenBank)
  • probably oxidises C-41 methyl branch in amphotericin precursor
Reference
ACC
PmId
[11451671] Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes. (Chem Biol. , 2001)
[16079135] Biosynthesis of amphotericin derivatives lacking exocyclic carboxyl groups. (J Biol Chem. , 2005)
comment
[PMID:11451671](2001)
Amphotericin生合成gene clusterの報告。
amphN: Cytochrome P450 monooxygenase

C-41 methyl分枝→carboxyl基形成に関与しそう。
amphN破壊mutant作成を試みているがうまくいっていない。

--
[PMID:16079135](2005)
amphN cytochrome P450 gene deletion mutantは、C-16環外carboxyl基がmethyl基で置換されているAmphotericin類似体を産生。この化合物は抗真菌活性を保持しているが、溶血作用は減少。

close this sectionSequence

selected fasta
>cytochrome P450 [AmphN]
MTAETEMTTFAPGCPVAFPLRRPGRPFPPPEYADYRAGEGLVRSELPASGPVWLVTRHED
VRTVLTDPRISADPSRPGFPRARRTGGAPSQSEIPGWFVALDPPEHDRFRKTLIPEFTVR
KVRELRPAIQQIVDERIDALLAAGNSADLIADFALSVPSLVISDLLGVPKADRDFFEAKT
KVLVTLSSTDEQRDEASKALLRYLNRLIQIKGRRPGEDLISRLLQAGTMNRQELSGVSML
LLIAGHETTANNIGLGVVQLLTNPQWIGDDRIVEEMLRYYSVADLVSFRVAVEDVEIGGQ
LIKAGEGIVPLIAAANHDGSVFDKPEEFNPERSARSHVAFGYGVHQCLGQNLVRVEMEIA
YRTLFERIPTLELAVPVEELPLKYDGVLFGLHELPVTWS
selected fasta
>cytochrome P450 [AmphN]
ATGACCGCCGAGACCGAGATGACGACCTTCGCGCCGGGCTGCCCCGTCGCCTTCCCGCTG
CGCCGGCCGGGACGTCCGTTCCCGCCGCCGGAGTACGCCGACTACCGTGCGGGCGAGGGC
CTGGTCCGCTCCGAACTGCCCGCAAGCGGCCCGGTGTGGCTGGTGACGCGGCACGAGGAC
GTGCGGACCGTGCTGACGGACCCCCGGATCAGCGCGGACCCCTCCCGCCCCGGCTTCCCG
AGGGCCAGGCGCACCGGCGGCGCCCCCTCGCAGTCGGAGATCCCGGGCTGGTTCGTGGCG
CTGGACCCCCCGGAGCACGACCGGTTCCGCAAGACGCTGATCCCGGAGTTCACCGTGCGC
AAGGTGCGGGAGCTGCGTCCGGCGATCCAGCAGATCGTGGACGAGCGGATCGACGCGTTG
CTCGCCGCAGGCAACTCGGCGGACCTGATCGCGGACTTCGCGCTGTCCGTGCCGTCGCTG
GTCATCTCCGACCTGCTCGGGGTGCCCAAGGCGGACCGGGACTTCTTCGAGGCGAAGACA
AAGGTCCTGGTGACCCTCAGCTCCACCGACGAGCAACGTGACGAGGCCTCCAAGGCGCTG
CTGCGTTATCTGAACCGGCTCATCCAGATCAAGGGCCGCCGCCCCGGCGAGGACCTGATC
AGCCGGCTGCTCCAGGCCGGCACCATGAACCGGCAGGAGCTGTCCGGGGTGTCGATGCTC
CTGCTCATCGCGGGGCACGAGACCACGGCGAACAACATCGGCCTGGGCGTCGTCCAGTTG
CTGACCAACCCGCAGTGGATCGGCGACGACCGCATCGTGGAGGAGATGCTGCGGTACTAC
TCGGTCGCCGACCTGGTGTCCTTCCGGGTGGCCGTGGAGGACGTGGAGATCGGCGGCCAG
CTGATCAAGGCCGGTGAGGGCATCGTGCCGCTGATCGCCGCGGCCAATCACGACGGTTCG
GTCTTCGACAAGCCCGAGGAGTTCAACCCGGAGCGTTCCGCGCGCTCGCACGTGGCGTTC
GGGTACGGCGTCCACCAGTGCCTCGGGCAGAACCTGGTGCGGGTGGAGATGGAGATCGCC
TACCGTACCCTGTTCGAGCGCATCCCGACCCTCGAACTGGCGGTGCCGGTCGAGGAGTTG
CCCCTGAAGTACGACGGCGTGCTGTTCGGCCTGCACGAGCTGCCCGTCACATGGAGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[244-261] 3.1999989904635e-08 PR00385 [271-282] 3.1999989904635e-08 PR00385 [338-347] 3.1999989904635e-08 PR00385 [347-358] 3.1999989904635e-08 PR00385
PR00385   P450
[23-398] 2.50000000000001e-99 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[15-398] 9.09992432541739e-93 SSF48264
SSF48264   Cytochrome_P450
[271-367] 1.69999999999999e-21 PF00067
PF00067   p450
IPR002397 Cytochrome P450, B-class (Family)
[101-112] 1.20000117458134e-48 PR00359 [148-164] 1.20000117458134e-48 PR00359 [165-180] 1.20000117458134e-48 PR00359 [202-224] 1.20000117458134e-48 PR00359 [289-316] 1.20000117458134e-48 PR00359 [317-332] 1.20000117458134e-48 PR00359 [338-347] 1.20000117458134e-48 PR00359 [347-358] 1.20000117458134e-48 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[340-349] PS00086
PS00086   CYTOCHROME_P450
SignalP
[1-33] 0.317 Signal
Bacteria, Gram-positive   
[1-33] 0.159 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Amph_00110 Amph_00110   Amph_00130 Amph_00130
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